Identification of a conditionally essential heat shock protein in Escherichia coli.
نویسندگان
چکیده
Protein D48.5 was recognized as a heat-inducible protein of Escherichia coli during the screening of a group of random, temperature-inducible Mud-Lac fusion mutants. Physiological and genetic analysis demonstrated that (i) the structural gene for this protein, designated htpI, is a member of the sigma 32-dependent heat shock regulon, (ii) at 37 degrees C the synthesis of protein D48.5 is nearly constitutive, increasing slightly with growth rate in media of different composition, and (iii) this protein is essential for growth at high temperature.
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ورودعنوان ژورنال:
- Biochimica et biophysica acta
دوره 1207 2 شماره
صفحات -
تاریخ انتشار 1994